Virus:Article Title: Pharmacological administration of FGF21 reverses obesity through a parabrachial-projecting neuron population in the hindbrain.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HA-Tag (C29F4) Rabbit Monoclonal Antibody Cell signaling 3724 c-Fos (9F6) Rabbit Monoclonal Antibody Cell signaling 2250 Alexa FluorTM 568 goat anti-rabbit IgG (H + L) Invitrogen 3154584 Alexa FluorTM 488 goat anti-rabbit IgG (H + L) Invitrogen 3192622 Bacterial and virus strains AAV2/9CMVCreEGFP University of Iowa viral vector core N/A AAV2/9CMVeGFP University of Iowa viral vector core N/A AAV-hSyn-DIO-hM3Dq(Gq)-mCherry Addgene Cat# 44361-AAV2 AAV-hSyn-DIO-mCherry Addgene Cat# 50459-AAV2 AAV-hSyn-DIO-EGFP Addgene Cat# 50457-AAVrg AAV-FLEX-tdTomato Addgene Cat# 28306 AAV-EF1a-Flpo Addgene Cat# 55637-AAVrg AAV-EF1a-fDIO-Cre Addgene Cat# 121675-AAV9 Chemicals, peptides, and recombinant proteins Recombinant hFGF21 NovoNordisk N/A PF-05231023 Pfizer N/A CNQX Tocris Cat#: 0190 D-AP5 Tocris Cat#: 0106 PTX Tocris Cat#: 1128 Deschloroclozapine dihydrochloride (DCZ) (water soluble) Hello Bio HB9126 Clozapine-N-oxide Tocris Cat#: 4936 Gill’s Hematoxylin I StatLab SL97-16 PINT Ammonium hydroxide solution Sigma 320145 Critical commercial assays Fibroblast Growth Factor 21 Human ELISA BioVendor Cat#: RD191108200R Ultra Sensitive Mouse Insulin ELISA Crystal Chem 90080 Infinity Triglyceride Infinity, Thermo Scientific TR13421 Verichem Labs Matrix Plus Chemistry Reference Kit Fisher Scientific NC9592194 High-Capacity cDNA Reverse Transcription Kit Life Technologies 4368814 SYBR green SYBR Greener QPCR for ABI 2000rxns Thermofisher 1176002K Autokit Glucose Wako Diagnostics 439–90901 RNAscope 2.5 HD Detection Reagent -RED Advanced Cell Diagnostics 322360 RNAscope H2O2 and Protease Reagents Advanced Cell Diagnostics 322381 RNAscope Wash Buffer Reagents Advanced Cell Diagnostics 310091 RNAscope Target Retrieval Reagents Advanced Cell Diagnostics 322000 RNAscope Positive control probe Mm PPIB Advanced Cell Diagnostics 313911 RNAscope Negative control probe Mm DapB Advanced Cell Diagnostics 310043 RNAscope Probe - Mm-Klb-O1 Advanced Cell Diagnostics 481211 (Continued on next page) Cell Reports 45, 117093, April 28, 2026 15 .. The following mice were utilized in these studies: Nkx2.1-Cre (JAX 008661), KLBfl/fl,18 KLB loxTB,50 KLB-Cre; Vglut2-flp-Ai65,29 and KLB-Cre.31 All mice used in experiments were males on a C57BL/6J genetic background, except for KLB loxTB male mice which were on a mixed background (C57BL6/129Sv).
Plasmid Preparation:Article Title: Pharmacological administration of FGF21 reverses obesity through a parabrachial-projecting neuron population in the hindbrain.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HA-Tag (C29F4) Rabbit Monoclonal Antibody Cell signaling 3724 c-Fos (9F6) Rabbit Monoclonal Antibody Cell signaling 2250 Alexa FluorTM 568 goat anti-rabbit IgG (H + L) Invitrogen 3154584 Alexa FluorTM 488 goat anti-rabbit IgG (H + L) Invitrogen 3192622 Bacterial and virus strains AAV2/9CMVCreEGFP University of Iowa viral vector core N/A AAV2/9CMVeGFP University of Iowa viral vector core N/A AAV-hSyn-DIO-hM3Dq(Gq)-mCherry Addgene Cat# 44361-AAV2 AAV-hSyn-DIO-mCherry Addgene Cat# 50459-AAV2 AAV-hSyn-DIO-EGFP Addgene Cat# 50457-AAVrg AAV-FLEX-tdTomato Addgene Cat# 28306 AAV-EF1a-Flpo Addgene Cat# 55637-AAVrg AAV-EF1a-fDIO-Cre Addgene Cat# 121675-AAV9 Chemicals, peptides, and recombinant proteins Recombinant hFGF21 NovoNordisk N/A PF-05231023 Pfizer N/A CNQX Tocris Cat#: 0190 D-AP5 Tocris Cat#: 0106 PTX Tocris Cat#: 1128 Deschloroclozapine dihydrochloride (DCZ) (water soluble) Hello Bio HB9126 Clozapine-N-oxide Tocris Cat#: 4936 Gill’s Hematoxylin I StatLab SL97-16 PINT Ammonium hydroxide solution Sigma 320145 Critical commercial assays Fibroblast Growth Factor 21 Human ELISA BioVendor Cat#: RD191108200R Ultra Sensitive Mouse Insulin ELISA Crystal Chem 90080 Infinity Triglyceride Infinity, Thermo Scientific TR13421 Verichem Labs Matrix Plus Chemistry Reference Kit Fisher Scientific NC9592194 High-Capacity cDNA Reverse Transcription Kit Life Technologies 4368814 SYBR green SYBR Greener QPCR for ABI 2000rxns Thermofisher 1176002K Autokit Glucose Wako Diagnostics 439–90901 RNAscope 2.5 HD Detection Reagent -RED Advanced Cell Diagnostics 322360 RNAscope H2O2 and Protease Reagents Advanced Cell Diagnostics 322381 RNAscope Wash Buffer Reagents Advanced Cell Diagnostics 310091 RNAscope Target Retrieval Reagents Advanced Cell Diagnostics 322000 RNAscope Positive control probe Mm PPIB Advanced Cell Diagnostics 313911 RNAscope Negative control probe Mm DapB Advanced Cell Diagnostics 310043 RNAscope Probe - Mm-Klb-O1 Advanced Cell Diagnostics 481211 (Continued on next page) Cell Reports 45, 117093, April 28, 2026 15 .. The following mice were utilized in these studies: Nkx2.1-Cre (JAX 008661), KLBfl/fl,18 KLB loxTB,50 KLB-Cre; Vglut2-flp-Ai65,29 and KLB-Cre.31 All mice used in experiments were males on a C57BL/6J genetic background, except for KLB loxTB male mice which were on a mixed background (C57BL6/129Sv).
Bioprocessing:Article Title: Pharmacological administration of FGF21 reverses obesity through a parabrachial-projecting neuron population in the hindbrain.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HA-Tag (C29F4) Rabbit Monoclonal Antibody Cell signaling 3724 c-Fos (9F6) Rabbit Monoclonal Antibody Cell signaling 2250 Alexa FluorTM 568 goat anti-rabbit IgG (H + L) Invitrogen 3154584 Alexa FluorTM 488 goat anti-rabbit IgG (H + L) Invitrogen 3192622 Bacterial and virus strains AAV2/9CMVCreEGFP University of Iowa viral vector core N/A AAV2/9CMVeGFP University of Iowa viral vector core N/A AAV-hSyn-DIO-hM3Dq(Gq)-mCherry Addgene Cat# 44361-AAV2 AAV-hSyn-DIO-mCherry Addgene Cat# 50459-AAV2 AAV-hSyn-DIO-EGFP Addgene Cat# 50457-AAVrg AAV-FLEX-tdTomato Addgene Cat# 28306 AAV-EF1a-Flpo Addgene Cat# 55637-AAVrg AAV-EF1a-fDIO-Cre Addgene Cat# 121675-AAV9 Chemicals, peptides, and recombinant proteins Recombinant hFGF21 NovoNordisk N/A PF-05231023 Pfizer N/A CNQX Tocris Cat#: 0190 D-AP5 Tocris Cat#: 0106 PTX Tocris Cat#: 1128 Deschloroclozapine dihydrochloride (DCZ) (water soluble) Hello Bio HB9126 Clozapine-N-oxide Tocris Cat#: 4936 Gill’s Hematoxylin I StatLab SL97-16 PINT Ammonium hydroxide solution Sigma 320145 Critical commercial assays Fibroblast Growth Factor 21 Human ELISA BioVendor Cat#: RD191108200R Ultra Sensitive Mouse Insulin ELISA Crystal Chem 90080 Infinity Triglyceride Infinity, Thermo Scientific TR13421 Verichem Labs Matrix Plus Chemistry Reference Kit Fisher Scientific NC9592194 High-Capacity cDNA Reverse Transcription Kit Life Technologies 4368814 SYBR green SYBR Greener QPCR for ABI 2000rxns Thermofisher 1176002K Autokit Glucose Wako Diagnostics 439–90901 RNAscope 2.5 HD Detection Reagent -RED Advanced Cell Diagnostics 322360 RNAscope H2O2 and Protease Reagents Advanced Cell Diagnostics 322381 RNAscope Wash Buffer Reagents Advanced Cell Diagnostics 310091 RNAscope Target Retrieval Reagents Advanced Cell Diagnostics 322000 RNAscope Positive control probe Mm PPIB Advanced Cell Diagnostics 313911 RNAscope Negative control probe Mm DapB Advanced Cell Diagnostics 310043 RNAscope Probe - Mm-Klb-O1 Advanced Cell Diagnostics 481211 (Continued on next page) Cell Reports 45, 117093, April 28, 2026 15 .. The following mice were utilized in these studies: Nkx2.1-Cre (JAX 008661), KLBfl/fl,18 KLB loxTB,50 KLB-Cre; Vglut2-flp-Ai65,29 and KLB-Cre.31 All mice used in experiments were males on a C57BL/6J genetic background, except for KLB loxTB male mice which were on a mixed background (C57BL6/129Sv).
Recombinant:Article Title: Pharmacological administration of FGF21 reverses obesity through a parabrachial-projecting neuron population in the hindbrain.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HA-Tag (C29F4) Rabbit Monoclonal Antibody Cell signaling 3724 c-Fos (9F6) Rabbit Monoclonal Antibody Cell signaling 2250 Alexa FluorTM 568 goat anti-rabbit IgG (H + L) Invitrogen 3154584 Alexa FluorTM 488 goat anti-rabbit IgG (H + L) Invitrogen 3192622 Bacterial and virus strains AAV2/9CMVCreEGFP University of Iowa viral vector core N/A AAV2/9CMVeGFP University of Iowa viral vector core N/A AAV-hSyn-DIO-hM3Dq(Gq)-mCherry Addgene Cat# 44361-AAV2 AAV-hSyn-DIO-mCherry Addgene Cat# 50459-AAV2 AAV-hSyn-DIO-EGFP Addgene Cat# 50457-AAVrg AAV-FLEX-tdTomato Addgene Cat# 28306 AAV-EF1a-Flpo Addgene Cat# 55637-AAVrg AAV-EF1a-fDIO-Cre Addgene Cat# 121675-AAV9 Chemicals, peptides, and recombinant proteins Recombinant hFGF21 NovoNordisk N/A PF-05231023 Pfizer N/A CNQX Tocris Cat#: 0190 D-AP5 Tocris Cat#: 0106 PTX Tocris Cat#: 1128 Deschloroclozapine dihydrochloride (DCZ) (water soluble) Hello Bio HB9126 Clozapine-N-oxide Tocris Cat#: 4936 Gill’s Hematoxylin I StatLab SL97-16 PINT Ammonium hydroxide solution Sigma 320145 Critical commercial assays Fibroblast Growth Factor 21 Human ELISA BioVendor Cat#: RD191108200R Ultra Sensitive Mouse Insulin ELISA Crystal Chem 90080 Infinity Triglyceride Infinity, Thermo Scientific TR13421 Verichem Labs Matrix Plus Chemistry Reference Kit Fisher Scientific NC9592194 High-Capacity cDNA Reverse Transcription Kit Life Technologies 4368814 SYBR green SYBR Greener QPCR for ABI 2000rxns Thermofisher 1176002K Autokit Glucose Wako Diagnostics 439–90901 RNAscope 2.5 HD Detection Reagent -RED Advanced Cell Diagnostics 322360 RNAscope H2O2 and Protease Reagents Advanced Cell Diagnostics 322381 RNAscope Wash Buffer Reagents Advanced Cell Diagnostics 310091 RNAscope Target Retrieval Reagents Advanced Cell Diagnostics 322000 RNAscope Positive control probe Mm PPIB Advanced Cell Diagnostics 313911 RNAscope Negative control probe Mm DapB Advanced Cell Diagnostics 310043 RNAscope Probe - Mm-Klb-O1 Advanced Cell Diagnostics 481211 (Continued on next page) Cell Reports 45, 117093, April 28, 2026 15 .. The following mice were utilized in these studies: Nkx2.1-Cre (JAX 008661), KLBfl/fl,18 KLB loxTB,50 KLB-Cre; Vglut2-flp-Ai65,29 and KLB-Cre.31 All mice used in experiments were males on a C57BL/6J genetic background, except for KLB loxTB male mice which were on a mixed background (C57BL6/129Sv).
Enzyme-linked Immunosorbent Assay:Article Title: Pharmacological administration of FGF21 reverses obesity through a parabrachial-projecting neuron population in the hindbrain.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HA-Tag (C29F4) Rabbit Monoclonal Antibody Cell signaling 3724 c-Fos (9F6) Rabbit Monoclonal Antibody Cell signaling 2250 Alexa FluorTM 568 goat anti-rabbit IgG (H + L) Invitrogen 3154584 Alexa FluorTM 488 goat anti-rabbit IgG (H + L) Invitrogen 3192622 Bacterial and virus strains AAV2/9CMVCreEGFP University of Iowa viral vector core N/A AAV2/9CMVeGFP University of Iowa viral vector core N/A AAV-hSyn-DIO-hM3Dq(Gq)-mCherry Addgene Cat# 44361-AAV2 AAV-hSyn-DIO-mCherry Addgene Cat# 50459-AAV2 AAV-hSyn-DIO-EGFP Addgene Cat# 50457-AAVrg AAV-FLEX-tdTomato Addgene Cat# 28306 AAV-EF1a-Flpo Addgene Cat# 55637-AAVrg AAV-EF1a-fDIO-Cre Addgene Cat# 121675-AAV9 Chemicals, peptides, and recombinant proteins Recombinant hFGF21 NovoNordisk N/A PF-05231023 Pfizer N/A CNQX Tocris Cat#: 0190 D-AP5 Tocris Cat#: 0106 PTX Tocris Cat#: 1128 Deschloroclozapine dihydrochloride (DCZ) (water soluble) Hello Bio HB9126 Clozapine-N-oxide Tocris Cat#: 4936 Gill’s Hematoxylin I StatLab SL97-16 PINT Ammonium hydroxide solution Sigma 320145 Critical commercial assays Fibroblast Growth Factor 21 Human ELISA BioVendor Cat#: RD191108200R Ultra Sensitive Mouse Insulin ELISA Crystal Chem 90080 Infinity Triglyceride Infinity, Thermo Scientific TR13421 Verichem Labs Matrix Plus Chemistry Reference Kit Fisher Scientific NC9592194 High-Capacity cDNA Reverse Transcription Kit Life Technologies 4368814 SYBR green SYBR Greener QPCR for ABI 2000rxns Thermofisher 1176002K Autokit Glucose Wako Diagnostics 439–90901 RNAscope 2.5 HD Detection Reagent -RED Advanced Cell Diagnostics 322360 RNAscope H2O2 and Protease Reagents Advanced Cell Diagnostics 322381 RNAscope Wash Buffer Reagents Advanced Cell Diagnostics 310091 RNAscope Target Retrieval Reagents Advanced Cell Diagnostics 322000 RNAscope Positive control probe Mm PPIB Advanced Cell Diagnostics 313911 RNAscope Negative control probe Mm DapB Advanced Cell Diagnostics 310043 RNAscope Probe - Mm-Klb-O1 Advanced Cell Diagnostics 481211 (Continued on next page) Cell Reports 45, 117093, April 28, 2026 15 .. The following mice were utilized in these studies: Nkx2.1-Cre (JAX 008661), KLBfl/fl,18 KLB loxTB,50 KLB-Cre; Vglut2-flp-Ai65,29 and KLB-Cre.31 All mice used in experiments were males on a C57BL/6J genetic background, except for KLB loxTB male mice which were on a mixed background (C57BL6/129Sv).
Reverse Transcription:Article Title: Pharmacological administration of FGF21 reverses obesity through a parabrachial-projecting neuron population in the hindbrain.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HA-Tag (C29F4) Rabbit Monoclonal Antibody Cell signaling 3724 c-Fos (9F6) Rabbit Monoclonal Antibody Cell signaling 2250 Alexa FluorTM 568 goat anti-rabbit IgG (H + L) Invitrogen 3154584 Alexa FluorTM 488 goat anti-rabbit IgG (H + L) Invitrogen 3192622 Bacterial and virus strains AAV2/9CMVCreEGFP University of Iowa viral vector core N/A AAV2/9CMVeGFP University of Iowa viral vector core N/A AAV-hSyn-DIO-hM3Dq(Gq)-mCherry Addgene Cat# 44361-AAV2 AAV-hSyn-DIO-mCherry Addgene Cat# 50459-AAV2 AAV-hSyn-DIO-EGFP Addgene Cat# 50457-AAVrg AAV-FLEX-tdTomato Addgene Cat# 28306 AAV-EF1a-Flpo Addgene Cat# 55637-AAVrg AAV-EF1a-fDIO-Cre Addgene Cat# 121675-AAV9 Chemicals, peptides, and recombinant proteins Recombinant hFGF21 NovoNordisk N/A PF-05231023 Pfizer N/A CNQX Tocris Cat#: 0190 D-AP5 Tocris Cat#: 0106 PTX Tocris Cat#: 1128 Deschloroclozapine dihydrochloride (DCZ) (water soluble) Hello Bio HB9126 Clozapine-N-oxide Tocris Cat#: 4936 Gill’s Hematoxylin I StatLab SL97-16 PINT Ammonium hydroxide solution Sigma 320145 Critical commercial assays Fibroblast Growth Factor 21 Human ELISA BioVendor Cat#: RD191108200R Ultra Sensitive Mouse Insulin ELISA Crystal Chem 90080 Infinity Triglyceride Infinity, Thermo Scientific TR13421 Verichem Labs Matrix Plus Chemistry Reference Kit Fisher Scientific NC9592194 High-Capacity cDNA Reverse Transcription Kit Life Technologies 4368814 SYBR green SYBR Greener QPCR for ABI 2000rxns Thermofisher 1176002K Autokit Glucose Wako Diagnostics 439–90901 RNAscope 2.5 HD Detection Reagent -RED Advanced Cell Diagnostics 322360 RNAscope H2O2 and Protease Reagents Advanced Cell Diagnostics 322381 RNAscope Wash Buffer Reagents Advanced Cell Diagnostics 310091 RNAscope Target Retrieval Reagents Advanced Cell Diagnostics 322000 RNAscope Positive control probe Mm PPIB Advanced Cell Diagnostics 313911 RNAscope Negative control probe Mm DapB Advanced Cell Diagnostics 310043 RNAscope Probe - Mm-Klb-O1 Advanced Cell Diagnostics 481211 (Continued on next page) Cell Reports 45, 117093, April 28, 2026 15 .. The following mice were utilized in these studies: Nkx2.1-Cre (JAX 008661), KLBfl/fl,18 KLB loxTB,50 KLB-Cre; Vglut2-flp-Ai65,29 and KLB-Cre.31 All mice used in experiments were males on a C57BL/6J genetic background, except for KLB loxTB male mice which were on a mixed background (C57BL6/129Sv).
SYBR Green Assay:Article Title: Pharmacological administration of FGF21 reverses obesity through a parabrachial-projecting neuron population in the hindbrain.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HA-Tag (C29F4) Rabbit Monoclonal Antibody Cell signaling 3724 c-Fos (9F6) Rabbit Monoclonal Antibody Cell signaling 2250 Alexa FluorTM 568 goat anti-rabbit IgG (H + L) Invitrogen 3154584 Alexa FluorTM 488 goat anti-rabbit IgG (H + L) Invitrogen 3192622 Bacterial and virus strains AAV2/9CMVCreEGFP University of Iowa viral vector core N/A AAV2/9CMVeGFP University of Iowa viral vector core N/A AAV-hSyn-DIO-hM3Dq(Gq)-mCherry Addgene Cat# 44361-AAV2 AAV-hSyn-DIO-mCherry Addgene Cat# 50459-AAV2 AAV-hSyn-DIO-EGFP Addgene Cat# 50457-AAVrg AAV-FLEX-tdTomato Addgene Cat# 28306 AAV-EF1a-Flpo Addgene Cat# 55637-AAVrg AAV-EF1a-fDIO-Cre Addgene Cat# 121675-AAV9 Chemicals, peptides, and recombinant proteins Recombinant hFGF21 NovoNordisk N/A PF-05231023 Pfizer N/A CNQX Tocris Cat#: 0190 D-AP5 Tocris Cat#: 0106 PTX Tocris Cat#: 1128 Deschloroclozapine dihydrochloride (DCZ) (water soluble) Hello Bio HB9126 Clozapine-N-oxide Tocris Cat#: 4936 Gill’s Hematoxylin I StatLab SL97-16 PINT Ammonium hydroxide solution Sigma 320145 Critical commercial assays Fibroblast Growth Factor 21 Human ELISA BioVendor Cat#: RD191108200R Ultra Sensitive Mouse Insulin ELISA Crystal Chem 90080 Infinity Triglyceride Infinity, Thermo Scientific TR13421 Verichem Labs Matrix Plus Chemistry Reference Kit Fisher Scientific NC9592194 High-Capacity cDNA Reverse Transcription Kit Life Technologies 4368814 SYBR green SYBR Greener QPCR for ABI 2000rxns Thermofisher 1176002K Autokit Glucose Wako Diagnostics 439–90901 RNAscope 2.5 HD Detection Reagent -RED Advanced Cell Diagnostics 322360 RNAscope H2O2 and Protease Reagents Advanced Cell Diagnostics 322381 RNAscope Wash Buffer Reagents Advanced Cell Diagnostics 310091 RNAscope Target Retrieval Reagents Advanced Cell Diagnostics 322000 RNAscope Positive control probe Mm PPIB Advanced Cell Diagnostics 313911 RNAscope Negative control probe Mm DapB Advanced Cell Diagnostics 310043 RNAscope Probe - Mm-Klb-O1 Advanced Cell Diagnostics 481211 (Continued on next page) Cell Reports 45, 117093, April 28, 2026 15 .. The following mice were utilized in these studies: Nkx2.1-Cre (JAX 008661), KLBfl/fl,18 KLB loxTB,50 KLB-Cre; Vglut2-flp-Ai65,29 and KLB-Cre.31 All mice used in experiments were males on a C57BL/6J genetic background, except for KLB loxTB male mice which were on a mixed background (C57BL6/129Sv).
RNAscope:Article Title: Pharmacological administration of FGF21 reverses obesity through a parabrachial-projecting neuron population in the hindbrain.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HA-Tag (C29F4) Rabbit Monoclonal Antibody Cell signaling 3724 c-Fos (9F6) Rabbit Monoclonal Antibody Cell signaling 2250 Alexa FluorTM 568 goat anti-rabbit IgG (H + L) Invitrogen 3154584 Alexa FluorTM 488 goat anti-rabbit IgG (H + L) Invitrogen 3192622 Bacterial and virus strains AAV2/9CMVCreEGFP University of Iowa viral vector core N/A AAV2/9CMVeGFP University of Iowa viral vector core N/A AAV-hSyn-DIO-hM3Dq(Gq)-mCherry Addgene Cat# 44361-AAV2 AAV-hSyn-DIO-mCherry Addgene Cat# 50459-AAV2 AAV-hSyn-DIO-EGFP Addgene Cat# 50457-AAVrg AAV-FLEX-tdTomato Addgene Cat# 28306 AAV-EF1a-Flpo Addgene Cat# 55637-AAVrg AAV-EF1a-fDIO-Cre Addgene Cat# 121675-AAV9 Chemicals, peptides, and recombinant proteins Recombinant hFGF21 NovoNordisk N/A PF-05231023 Pfizer N/A CNQX Tocris Cat#: 0190 D-AP5 Tocris Cat#: 0106 PTX Tocris Cat#: 1128 Deschloroclozapine dihydrochloride (DCZ) (water soluble) Hello Bio HB9126 Clozapine-N-oxide Tocris Cat#: 4936 Gill’s Hematoxylin I StatLab SL97-16 PINT Ammonium hydroxide solution Sigma 320145 Critical commercial assays Fibroblast Growth Factor 21 Human ELISA BioVendor Cat#: RD191108200R Ultra Sensitive Mouse Insulin ELISA Crystal Chem 90080 Infinity Triglyceride Infinity, Thermo Scientific TR13421 Verichem Labs Matrix Plus Chemistry Reference Kit Fisher Scientific NC9592194 High-Capacity cDNA Reverse Transcription Kit Life Technologies 4368814 SYBR green SYBR Greener QPCR for ABI 2000rxns Thermofisher 1176002K Autokit Glucose Wako Diagnostics 439–90901 RNAscope 2.5 HD Detection Reagent -RED Advanced Cell Diagnostics 322360 RNAscope H2O2 and Protease Reagents Advanced Cell Diagnostics 322381 RNAscope Wash Buffer Reagents Advanced Cell Diagnostics 310091 RNAscope Target Retrieval Reagents Advanced Cell Diagnostics 322000 RNAscope Positive control probe Mm PPIB Advanced Cell Diagnostics 313911 RNAscope Negative control probe Mm DapB Advanced Cell Diagnostics 310043 RNAscope Probe - Mm-Klb-O1 Advanced Cell Diagnostics 481211 (Continued on next page) Cell Reports 45, 117093, April 28, 2026 15 .. The following mice were utilized in these studies: Nkx2.1-Cre (JAX 008661), KLBfl/fl,18 KLB loxTB,50 KLB-Cre; Vglut2-flp-Ai65,29 and KLB-Cre.31 All mice used in experiments were males on a C57BL/6J genetic background, except for KLB loxTB male mice which were on a mixed background (C57BL6/129Sv).
Positive Control:Article Title: Pharmacological administration of FGF21 reverses obesity through a parabrachial-projecting neuron population in the hindbrain.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HA-Tag (C29F4) Rabbit Monoclonal Antibody Cell signaling 3724 c-Fos (9F6) Rabbit Monoclonal Antibody Cell signaling 2250 Alexa FluorTM 568 goat anti-rabbit IgG (H + L) Invitrogen 3154584 Alexa FluorTM 488 goat anti-rabbit IgG (H + L) Invitrogen 3192622 Bacterial and virus strains AAV2/9CMVCreEGFP University of Iowa viral vector core N/A AAV2/9CMVeGFP University of Iowa viral vector core N/A AAV-hSyn-DIO-hM3Dq(Gq)-mCherry Addgene Cat# 44361-AAV2 AAV-hSyn-DIO-mCherry Addgene Cat# 50459-AAV2 AAV-hSyn-DIO-EGFP Addgene Cat# 50457-AAVrg AAV-FLEX-tdTomato Addgene Cat# 28306 AAV-EF1a-Flpo Addgene Cat# 55637-AAVrg AAV-EF1a-fDIO-Cre Addgene Cat# 121675-AAV9 Chemicals, peptides, and recombinant proteins Recombinant hFGF21 NovoNordisk N/A PF-05231023 Pfizer N/A CNQX Tocris Cat#: 0190 D-AP5 Tocris Cat#: 0106 PTX Tocris Cat#: 1128 Deschloroclozapine dihydrochloride (DCZ) (water soluble) Hello Bio HB9126 Clozapine-N-oxide Tocris Cat#: 4936 Gill’s Hematoxylin I StatLab SL97-16 PINT Ammonium hydroxide solution Sigma 320145 Critical commercial assays Fibroblast Growth Factor 21 Human ELISA BioVendor Cat#: RD191108200R Ultra Sensitive Mouse Insulin ELISA Crystal Chem 90080 Infinity Triglyceride Infinity, Thermo Scientific TR13421 Verichem Labs Matrix Plus Chemistry Reference Kit Fisher Scientific NC9592194 High-Capacity cDNA Reverse Transcription Kit Life Technologies 4368814 SYBR green SYBR Greener QPCR for ABI 2000rxns Thermofisher 1176002K Autokit Glucose Wako Diagnostics 439–90901 RNAscope 2.5 HD Detection Reagent -RED Advanced Cell Diagnostics 322360 RNAscope H2O2 and Protease Reagents Advanced Cell Diagnostics 322381 RNAscope Wash Buffer Reagents Advanced Cell Diagnostics 310091 RNAscope Target Retrieval Reagents Advanced Cell Diagnostics 322000 RNAscope Positive control probe Mm PPIB Advanced Cell Diagnostics 313911 RNAscope Negative control probe Mm DapB Advanced Cell Diagnostics 310043 RNAscope Probe - Mm-Klb-O1 Advanced Cell Diagnostics 481211 (Continued on next page) Cell Reports 45, 117093, April 28, 2026 15 .. The following mice were utilized in these studies: Nkx2.1-Cre (JAX 008661), KLBfl/fl,18 KLB loxTB,50 KLB-Cre; Vglut2-flp-Ai65,29 and KLB-Cre.31 All mice used in experiments were males on a C57BL/6J genetic background, except for KLB loxTB male mice which were on a mixed background (C57BL6/129Sv).
Negative Control:Article Title: Pharmacological administration of FGF21 reverses obesity through a parabrachial-projecting neuron population in the hindbrain.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HA-Tag (C29F4) Rabbit Monoclonal Antibody Cell signaling 3724 c-Fos (9F6) Rabbit Monoclonal Antibody Cell signaling 2250 Alexa FluorTM 568 goat anti-rabbit IgG (H + L) Invitrogen 3154584 Alexa FluorTM 488 goat anti-rabbit IgG (H + L) Invitrogen 3192622 Bacterial and virus strains AAV2/9CMVCreEGFP University of Iowa viral vector core N/A AAV2/9CMVeGFP University of Iowa viral vector core N/A AAV-hSyn-DIO-hM3Dq(Gq)-mCherry Addgene Cat# 44361-AAV2 AAV-hSyn-DIO-mCherry Addgene Cat# 50459-AAV2 AAV-hSyn-DIO-EGFP Addgene Cat# 50457-AAVrg AAV-FLEX-tdTomato Addgene Cat# 28306 AAV-EF1a-Flpo Addgene Cat# 55637-AAVrg AAV-EF1a-fDIO-Cre Addgene Cat# 121675-AAV9 Chemicals, peptides, and recombinant proteins Recombinant hFGF21 NovoNordisk N/A PF-05231023 Pfizer N/A CNQX Tocris Cat#: 0190 D-AP5 Tocris Cat#: 0106 PTX Tocris Cat#: 1128 Deschloroclozapine dihydrochloride (DCZ) (water soluble) Hello Bio HB9126 Clozapine-N-oxide Tocris Cat#: 4936 Gill’s Hematoxylin I StatLab SL97-16 PINT Ammonium hydroxide solution Sigma 320145 Critical commercial assays Fibroblast Growth Factor 21 Human ELISA BioVendor Cat#: RD191108200R Ultra Sensitive Mouse Insulin ELISA Crystal Chem 90080 Infinity Triglyceride Infinity, Thermo Scientific TR13421 Verichem Labs Matrix Plus Chemistry Reference Kit Fisher Scientific NC9592194 High-Capacity cDNA Reverse Transcription Kit Life Technologies 4368814 SYBR green SYBR Greener QPCR for ABI 2000rxns Thermofisher 1176002K Autokit Glucose Wako Diagnostics 439–90901 RNAscope 2.5 HD Detection Reagent -RED Advanced Cell Diagnostics 322360 RNAscope H2O2 and Protease Reagents Advanced Cell Diagnostics 322381 RNAscope Wash Buffer Reagents Advanced Cell Diagnostics 310091 RNAscope Target Retrieval Reagents Advanced Cell Diagnostics 322000 RNAscope Positive control probe Mm PPIB Advanced Cell Diagnostics 313911 RNAscope Negative control probe Mm DapB Advanced Cell Diagnostics 310043 RNAscope Probe - Mm-Klb-O1 Advanced Cell Diagnostics 481211 (Continued on next page) Cell Reports 45, 117093, April 28, 2026 15 .. The following mice were utilized in these studies: Nkx2.1-Cre (JAX 008661), KLBfl/fl,18 KLB loxTB,50 KLB-Cre; Vglut2-flp-Ai65,29 and KLB-Cre.31 All mice used in experiments were males on a C57BL/6J genetic background, except for KLB loxTB male mice which were on a mixed background (C57BL6/129Sv).
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